These datasets contain the results from satellite tracking the movements of Adelie Penguins (Pygoscelis adeliae) from the following locations in Antarctica: Bechervaise Island, Magnetic Island, Shirley Island, Edmonson Point in Terra Nova Bay, Dumont D'Urville area. By the use of satellite fixes the foraging locations of the penguins were determined. See the child metadata records for further information. This work was completed as part of ASAC project 2205 (ASAC_2205), 'Adelie penguin research and monitoring in support of the CCAMLR Ecosystem Monitoring Project'.

This metadata record contains the results from bioassays conducted to show the response of an Antarctic nemertean Antarctonemertes unilineata to contamination from combinations of Special Antarctic Blend (SAB) diesel, Marine Gas Oil (MGO) and Intermediate Fuel Oil (IFO 180), chemically dispersed with fuel dispersants Ardrox 6120, Slickgone LTSW and Slickgone NS. Note that the corresponding PhD thesis chapter refers to the species as Antarctonemertes sp., prior to being named Antarctonemertes unilineata in 2018. Experiments using SAB, MGO and IFO 180 with the dispersant Ardrox 6120, including fuel only and dispersant only treatments were conducted at Casey station. Experiments involving IFO 180 and the fuel dispersants Slickgone LTSW and Slickgone NS were conducted at the Antarctic Division’s Marine Research Facility quarantine labs. All experimental procedures, including test mix preparation and bioassays were conducted at 0 plus or minus 1 degree C. Water accommodated fractions (WAF; fuel mixed in water) and chemically enhanced water accommodated fractions (CEWAF) were made according to the specifications of Singer, Aurand et al. (2000), Barron and Ka’aihue (2003) and Kotzakoulakis (unpublished at time of writing). Dispersant only mixes were also made using filtered seawater (FSW) and dispersant volumes proportional to those used for CEWAF production. WAF was made using a loading ratio of 1: 25 (v/v) fuel to FSW, CEWAF was prepared using 1:100 (v/v) fuel to FSW ratio, and 1: 20 (v/v) dispersant to fuel ratio. Following the 48 h preparation time, the seawater WAF components of the mix were drained from the bottom of aspirator bottles and serially diluted. WAF treatment concentrations were 100%, 50%, 20% and 10%, CEWAF and dispersant only concentrations were 10%, 5%, 1% and 0.1%. Treatment solutions were replenished every four days to simulate a repeated pulse exposure to contaminants and to replace hydrocarbons lost through evaporation and adsorption and to maintain water quality parameters. WAF, CEWAF and dispersant only test solutions were remade every four days using identical methods. Tests were done in temperature-controlled cabinets set to 0 plus or minus 1 degree C following a 6 h light to 18 h dark photoperiod. Beakers were left uncovered to allow for the natural evaporation of lighter hydrocarbon components to reflect real fuel spill conditions. Experiments ran for 24 d except for the Ardrox 6120 only experiment, which ran for 16 d due to high mortality in this treatment. Sublethal and lethal endpoints were assessed at 1, 2, 4, 7, 8, 12, 14, 16, 20 and 24 d observations. Aliquot water samples for analysis of total hydrocarbon content (THC) were taken for initial and final test concentrations, and before and after each four-day water change, to obtain accurate profiles of hydrocarbon loss over the test period. Duplicate samples were taken for every treatment concentration and extracted with dichloromethane, spiked with an internal standard of 1-bromoeicosane and cyclooctane. Samples were analysed using gas chromatography with flame ionization detection (GC-FID) and gas chromatography mass spectrometry (GC-MS). Average THC concentrations for the duration of the experiment were obtained by integrating the measured concentrations to which animals were exposed following the methods of Brown et al. (2016) and Payne et al. (2014). This data submission includes one file detailing the TPH experiment analyses and one detailing the bioassay tests and results. The thesis that relates to this work is available from: https://epubs.scu.edu.au/theses/533/

A survey of macrobenthic assemblages in soft-sediments was done at Casey Station, East Antarctica. Samples were taken by divers using hand-held corers (core size - 10 cm diameter by 10 cm deep). This was the final component of a large nested sampling survey extending over a three year periods with samples taken in three summers and one winter period. The aims were: 1) To examine spatial variation at several scales in these assemblages; 2) To determine if there were differences between potentially impacted areas and control areas; 3) To determine the level of replication, taxonomic resolution and data transformation that are appropriate to studies of human impacts in Antarctic soft-sediment assemblages. Cores were collected by divers in a hierarchical, spatially nested design incorporating 4 scales: Locations (1000s of metres apart), Sites (100s of metres), Plots (10s of metres) and among replicates within plots (~1 metre). This data set consists of 48 core samples from three locations, O'Brien Bay, Sparkes Bay and Wilkes. Samples are sorted mainly to species. Links to ASAC 1100. The fields in this dataset are: Location Site/Rep Species

A survey of macrobenthic assemblages in soft-sediments was done at Casey Station, East Antarctica. Samples were taken along four transects in Brown Bay, along a pollution gradient that has its source at an abandoned waste tip on the shore of Brown Bay. The transects were up to 300 M long and there were 9 sampling stations on each transect except for transect four which had only 4 sampling stations. Sampling stations were at the following distances from the shoreline and tip site: 10 m, 20 m, 30 m, 40 m, 50 m, 100 m, 150 m, 200 m, and 300 m. Two cores of the soft-sediment assemblages were taken at each sampling station and two cores for analysis of heavy metals and hydrocarbons. Samples were taken by divers using hand-held corers (fauna core size - 10 cm diameter by 10 cm deep; sediment analysis cores - 5 cm diam. by 10 cm deep). The aims were: 1) to determine the nature and extent of the contamination gradient in front of the tip site and; 2) to determine if there was a corresponding response in the soft-sediment assemblages along the contamination gradient. A total of 62 samples of soft-sediment assemblages and 62 samples for heavy metal and hydrocarbon analysis were taken. Sediment grain size was also examined at each sampling point. The benthic diatom communities were also analysed in each sample. Links to ASAC 1100. The fields in this dataset are: Distance Position Site and replicate Species

The recruitment of epifauna (sessile and mobile) on hard-substratum was examined in a field experiment using tiles. A total of 160 tiles were deployed at five locations, with 32 tiles at each location, arranged in a spatially nested design. There were three potentially impacted locations locations (two in Brown Bay and one in Shannon Bay) and two control locations (in O'Brien Bay). This metadata record describes data from the first sampling time only. Eight tiles were collected from each location 15 months after the initial deployment. The experiment was setup so that recruitment of sessile epifauna to both the upper and lower sides of the tiles could be examined. The mobile epifauna on the tiles were also collected and are described in a separate metadata record. Heavy recruitment was observed on the underside of the tile and only light recruitment was observed on the upper surface. Also links to ASAC 1100.

The recruitment of mobile epifauna on hard-substratum was examined in a field experiment using tiles. A total of 160 tiles were deployed at five locations, with 32 tiles at each location, arranged in a spatially nested design. There were three potentially impacted locations locations (two in Brown Bay and one in Shannon Bay) and two control locations (in O'Brien Bay). This metadata record describes data from the first sampling time only. Eight tiles were collected from each location 15 months after the initial deployment. The experiment was setup so that the combined recruitment of mobile epifauna to the upper and lower sides of the tiles could be examined. The sessile epifauna on the tiles were also collected and are described in a separate metadata record. A total of 40 samples are included in this data. Also links to ASAC 1100.

The effects of hyrdocarbon and heavy metal contamination of marine sediments on recruitment of soft-sediment assemblages were examined in a field experiment at Casey Station, East Antarctica. Three locations were used, a polluted bay adjacent to an old disused tip site (Brown Bay) and two control locations (O'Brien Bay and Sparkes Bay). At each location three types of defaunated sediment (hydrocarbon treated, heavy metal treated and control) were placed at approximately 15 m depth and left in place for 3 months, from December to February. Sediments were artificially contaminated with hydrocarbons and metals at concentrations which were representative of levels found in sediments at contaminated sites around Casey Station. There were large differences in recruitment between the three locations and significant differences between the control and contaminated sediment. Sediments in the experiment were also examined for evidence of degradation and attenuation of hydrocarbons and heavy metals. A total of 104 recruitment samples were collected. Samples were sieved at 500 micro m and sorted mainly to species. Other work to arise from this experiment includes examination of the effects on diatom communities and microbial communities. Data includes fauna, metals and hydrocarbon concentrations in experiment. Pre-deployment concentrations (before experiment was deployed in water) are indicated as 'pre-deployment'. Concentrations of contaminants in sediments surrounding the experiment (within several metres) are indicated as 'surrounding'. This project also links to ASAC 1100. The fields in this dataset are: Location Site Treatment (tmt) Site and replicate Species Toxicity Arsenic Cadmium Copper Lead Silver Zinc Special Antarctic Blend Fuel (SAB) Lube TPH

The effect of location and sediment contamination on recruitment of soft-sediment assemblages were examined in field experiment at Casey Station, East Antarctica. Four locations were used, a polluted bay adjacent to an old disused tip site (Brown Bay), a bay adjacent to the Casey Station sewage outfall, and two undisturbed control locations in O'Brien Bay. At each location two types of defaunated sediment (polluted and control) were placed 12 - 18 m, in experimental trays. Half of the experimental sediments were left in place over the Austral winter, from March - November, and the remaining sediments were collected after a total of one year, in February 1999. There were large differences in recruitment between the two locations and significant differences between the polluted and control sediment. There were not only differences in abundance of taxa and assemblage structure but also in spatial variability and variability of populations of certain taxa, with recruitment to the control locations more variable than polluted locations, and recruitment in the control sediment more variable than the polluted sediment. The majority of fauna recruiting to the experiment were highly motile colonizing species with non-pelagic lecithotrophic larvae, usually brooded and released as dispersing juveniles, such as gammarids, tanaids, isopods and gastropods. A total of 64 recruitment samples were collected after 9 months and 52 samples after one year. Samples were sieved at 500 micro m and sorted mainly to species. Samples are rows in data sheet. Site codes include place name (e.g. BB2) and experimental treatment (e.g. C1 - control 1). See accompanying sheet for full details of codes, including species names. Sediment chemistry data are means (and standard errors) for each treatment (averaged over 2 trays). Also links to ASAC 1100. The fields in this dataset are: Species Site Sample Abundance Toxicity Arsenic Cadmium Copper Lead Silver Zinc

Marine soft-sediment assemblages were sampled from shallow (5 - 35m) nearshore regions around Casey Station, Windmill Islands, East Antarctica in winter 1998, using a van-Veen grab (surface area 20 x 25 cm). Samples were sieved through a 1 mm mesh and sorted to species where possible. A hierarchical, spatially nested sampling design was used with locations (km's apart), sites (100s of metres apart). Two potentially impacted, polluted locations (Shannon Bay - adjacent to a sewage outfall; and Brown Bay - adjacent to an old garbage tip) were compared with a control location in O'Brien Bay. Data were analysed using both multivariate and univariate statistical methods. Significant differences in assemblages were found between locations and between sites within locations. Significant differences in the abundances of taxa at several taxonomic levels (species, family, order, phylum) were also found at both spatial scales. Significant differences were also detected between the polluted and control locations. These samples were collected as part of a larger program examining human impacts in marine benthic assemblages at Casey Station. These samples were used in an analysis of temporal changes in soft-sediment assemblages at Casey. A total of 30 grab samples were collected in this survey. The fields in this dataset are: Location Site Date Site/Replicate Species

A survey of the epibenthic fauna of hard-substrata was made around Casey Station, using a camera mounted on a frame to take photoquadrats. A nested sampling design was used with several spatial scales. At each location there were two sites, approximately 50-100 m apart. At each site there were three by 15 m long transects, approximately 5 m apart. On each transect there were 2 plots, 5 m long by 2 m wide. Six photoquadrats were taken in each plot. This metadata record describes one component of this survey, photoquadrats taken in flat or gently sloping rocky areas, where the bottom consisted of flat bedrock, gravel or cobbles. A separate survey was done in and around boulders and boulder fields. One of the aims of this survey was to determine if there is any evidence of human impacts in these assemblages. There were several control and several potentially impacted locations. The data have yet to be analysed. Also links to ASAC 1100.